因为把双端的read需要判断方向,所以第一步的时候就已经用根据建库的方式,duTP建库第一条链做反向互补,在read后面加上/1,第二条链加上/2,SMART建库的时候,第二条链做反向互补加上/1,第二条链加上/2。因为做这个转化的时候在read名字后面多了一个space号,所以出现了上面类似的问题
@E00511:189:HC3TYCCXY:5:1101:3051:1959/1
GGGCATGGGATTTACTTATTCAGCAAAGGGTTGCCTTTACCACCCAGAAGGATTTTGGAAGAGAGGCCAGAGAGATAAGATGGGCTCATGCTCAGAGGACTTTGCATGGATTACAACCACCAGACACAAAACTGTTTGGTGACAGAA
+
<F7FJFFJJJAJJJFJJJJJJJJJFAJJJJJJJJJJJJJJJJJFFFFJJJJJA<A<JJJJJJJJJJJJJJJJJJJJJJJJJFJJJJJAJJJFJJJJJJJJFAAJFJFJJJ-FJFJJJ-A-FJJJJJJJJJJJJJJJJFJJFJJJJJJ
@E00511:189:HC3TYCCXY:5:1101:3133:1959/2
GGTGTAGCAGGAACTGGACCGTCCTCCGCGAGGTCTGATAATGCCTCGATTGGTGTATTGCCTGCTAATTTGTCTTCTAATAGAGAGAGCAAGCCCATTTCAGAGAACCCGGAAGAAGAGAACGATGGAGTCCGGGATATTCATGTA
+
FFJAJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFFJJJJJJJJJ7AJJFAFFJJF-FJJJJJJJJ<JJJJJJAJJJJFJJJJJJJJJJJ<JJJFJJJFJJJJJJJJJJFJJJJJJJFAFJJJJJJJJJJJFJJJJJJJJJFJJJJJJ